Isolation And Characterization Of Oil Degrading Bacteria Pdf

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30.11.2020 at 07:43
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isolation and characterization of oil degrading bacteria pdf

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Two yeast strains are enriched and isolated from industrial refinery wastewater.

Petroleum-Tolerant Rhizospheric Bacteria: Isolation, Characterization and Bioremediation Potential

Besides this direct pollution, the occasionally pipeline leaks, transportation accidents, storage tank ruptures and refining petroleum is further intensifying the pollution of this area. Crude petroleum and its associated products contain many kinds of organic compounds, dominated by aliphatic and aromatic hydrocarbons. Bacteria abundance, their fast growth and wide spectrum of molecules, have allowed them to be considered as a main bioremediation tool. Up to now, many bacterial strains, such as Pseudomonas , Alcanivorax , Acinetobacter , Rhodococcus , and Bacillus have been isolated from soil and water, and were effectively used for the bioremediation of petroleum contaminants. It is well established that many microbial catabolic pathways are involved in the degradation of petroleum 8. For example, the alk pathway in Rhodococcussp.

This study was aimed at isolating and characterizing hydrocarbon-degrading fungi from hydrocarbon-contaminated soil in Ogbe-Ijoh oil creek in Warri, Delta State, Nigeria. Isolation and characterization were carried out using standard methods. Biodegradation assay was carried out over 25 days and monitored using the following parameters; pH, total organic carbon, and total hydrocarbon content. The fungi isolated were: Penicillium sp. This showed the ability of the fungal isolates to utilize crude oil as carbon source, producing acid during its metabolism. The highest pH value during degradation by the fungal isolates was recorded in Fusarium sp. The fungal isolates were able to reduce the total carbon and hydrocarbon content during degradation.

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Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Petroleum is an important energy source. Due to its intensive exploration, accidents resulting in oil spills on soil are frequent, which creates consequences to ecosystems and human health.

PDF | The oil degrading bacterial strains were isolated from marine sediments collected from fuel oil–polluted coastal area in Penang, Malaysia.

Isolation and characterisation of crude oil sludge degrading bacteria

Scientific Research An Academic Publisher. Science of the Total Environment, 92, Indian Journal of Experimental Biology, 47, Oleaginous, Fatty Substance, Lipids, 12, Applied and Environmental Microbiology, 57,

The aim of this work was to isolate and characterize bacteria from two crude oil-contaminated soils which were capable of producing biosurfactant and degrade oil. The contaminated soils were located in Tehran and Isfahan oil refineries, Iran. Various traits, such as hemolytic activity, oil resolving , oil spreading, and oil emulsifying capability were used to screen the isolates. Among 54 isolated isolates from two petroleum-contaminated soils, only 10 isolates were able to produce biosurfactant and degrade oil.

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Metrics details. The use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly. Polycyclic aromatic hydrocarbons PAHs , as the major components of oil sludge, are hydrophobic and recalcitrant. An important way of enhancing the rate of PAH desorption is to compost crude oil sludge by incorporating commercial surfactants, thereby making them available for microbial degradation. Molecular characterisation of the 16S rRNA genes indicated that the isolates obtained on a mineral salts medium belonged to different genera, including Stenotrophmonas , Pseudomonas , Bordetella , Brucella , Bacillus , Achromobacter , Ochrobactrum , Advenella , Mycobacterium , Mesorhizobium , Klebsiella , Pusillimonas and Raoultella. The percentage degradation rates of these isolates were estimated by measuring the absorbance of the 2,6-dichlorophenol indophenol medium.

BioMed Research International

Water Sci Technol 29 December ; 78 12 : — The aim of this study was to isolate hydrocarbons-degrading bacteria for treatment of oily wastewater from long-standing petroleum-polluted sediments in Bohai Bay, China. Six hydrocarbons-degrading bacteria were screened and identified as Pseudomonas sp. A new approach using a combination of various bacterial species in petroleum biodegradation was proposed and evaluated for its degradation characteristics. Besides, after 14 days of culture, the biodegradation assessment markers, pristane and phytane, showed significant degradation rates of These results indicated that YJ01 could degrade a wider range of hydrocarbons as well as some recalcitrant hydrocarbon components, and can be applied for bioremediation and treatment of oil-contaminated environment. Petroleum hydrocarbons pollution PHP is becoming increasingly severe with the continuously growing demands for oil and other oil-related products.


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